Incidental identification of Strongyloides stercoralis infection by broad-range 28S rDNA gene sequencing in a patient with a hematolymphoid malignancy

Diagn Microbiol Infect Dis. 2016 Dec;86(4):362-364. doi: 10.1016/j.diagmicrobio.2016.08.029. Epub 2016 Sep 4.

Abstract

Strongyloides stercoralis is an important human parasite, especially in rural areas and developing countries. Infected immunosuppressed patients are at risk for hyperinfection, with severe clinical consequences. Here we describe the incidental detection and diagnosis of an unexpected S. stercoralis infection by methods designed to detect fungal 28S ribosomal DNA.

Keywords: 28S; Fungal; Helminth; Parasite; Strongyloides; rDNA.

Publication types

  • Case Reports

MeSH terms

  • Animals
  • Cluster Analysis
  • DNA, Helminth / chemistry
  • DNA, Helminth / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Hematologic Neoplasms / complications*
  • Humans
  • Male
  • Microscopy
  • Middle Aged
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 28S / genetics
  • Sequence Analysis, DNA
  • Strongyloides stercoralis / genetics
  • Strongyloides stercoralis / isolation & purification*
  • Strongyloidiasis / diagnosis*
  • Strongyloidiasis / parasitology
  • Strongyloidiasis / pathology*

Substances

  • DNA, Helminth
  • DNA, Ribosomal
  • RNA, Ribosomal, 28S