Flash photolysis of the serum albumin-heme-CO complex

Biochemistry. 1989 May 16;28(10):4422-6. doi: 10.1021/bi00436a045.

Abstract

Protoheme-CO in aqueous solution does not exhibit a geminate ligand recombination reaction. Addition of a protein, either globin or serum albumin, to which heme binds strongly, leads to an observable geminate reaction in aqueous solution. The bimolecular kinetic data for the albumin-heme-CO complex show two stable components, one heme-like in rate and difference spectrum, and one hemoglobin-like. The geminate reaction correlates spectrally with the hemoglobin-like component.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Carbon Monoxide / radiation effects*
  • Heme / radiation effects*
  • Kinetics
  • Photolysis
  • Serum Albumin / radiation effects*
  • Spectrophotometry

Substances

  • Serum Albumin
  • Heme
  • Carbon Monoxide