Chlorogenic acid a dietary polyphenol attenuates isoproterenol induced myocardial oxidative stress in rat myocardium: An in vivo study

Biomed Pharmacother. 2016 Dec:84:208-214. doi: 10.1016/j.biopha.2016.09.028. Epub 2016 Sep 19.

Abstract

Intent of the present study has been made to appraise the cardioprotective effect of chlorogenic acid (CGA) on isoproterenol (ISO) induced myocardial infarction (MI) in male albino Wistar rats. ISO-induced myocardial damage was indicated by the elevated levels of marker enzymes such as creatine kinase (CK), creatine kinase-MB (CK-MB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and troponin T and I (cTnT, cTnI) in the serum. In addition, the levels of lipid peroxidation products such as thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and lipid hydroperoxides (LHPs) were significantly increased in the plasma and heart tissue. Activities of enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and the non enzymic antioxidants like vitamin C, vitamin E and reduced glutathione (GSH) were decreased in the erythrocytes, plasma and heart tissue of the ISO-induced rats and myocardium infarct size as observed by staining with triphenyltetrazolium chloride (TTC). Histopathological observation corroborated with the bioochemical parameters. Oral administration of CGA at different doses (10, 20, 40mg/kg BW) for 19days prevented the above changes. The 40mg/kg BW of CGA was more pronounced than other two doses and brought back all the above parameters to near normalcy.

Keywords: Antioxidants; Chlorogenic acid; Isoproterenol; Lipid peroxidation products; Myocardial infarction.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Biomarkers / blood
  • Chlorogenic Acid / pharmacology*
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Isoproterenol*
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / metabolism
  • Myocardial Infarction / pathology
  • Myocardial Infarction / prevention & control*
  • Myocardium / metabolism*
  • Myocardium / pathology
  • Oxidative Stress / drug effects*
  • Rats, Wistar
  • Time Factors

Substances

  • Antioxidants
  • Biomarkers
  • Chlorogenic Acid
  • Isoproterenol