Joining DNA sequences to create linear and circular constructs is a basic requirement in molecular biology. Here we describe PaperClip, a recently developed method, which enables assembly of multiple DNA sequences in one reaction in a combinatorial manner. In contrast to other homology-based multi-part assembly methods currently available, PaperClip allows assembly of a given set of parts in any order without requiring specific single-use oligonucleotides for each assembly order.
Keywords: Agarose gel electrophoresis; CPEC; Cell extract; DNA assembly; Ligation; Molecular cloning; Oligonucleotide annealing; PAGE; PCR; SLiCE; Selection; Single-pot assembly.