Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Seung Mok Ryu; Jaeyoung Kwon; Young Hye Seo; Eun Gyeong Song; Seong Su Hong; Beom Seok Kim; Jin Sung Hong; Ki Hyun Ryu; Dongho Lee

doi:10.1016/j.virusres.2016.09.013

Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Virus Res. 2017 Jan 2:227:49-56. doi: 10.1016/j.virusres.2016.09.013. Epub 2016 Sep 26.

Authors

Seung Mok Ryu¹, Jaeyoung Kwon², Young Hye Seo¹, Eun Gyeong Song³, Seong Su Hong⁴, Beom Seok Kim¹, Jin Sung Hong⁵, Ki Hyun Ryu⁶, Dongho Lee⁷

Affiliations

¹ Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
² Natural Constituents Research Center, Korea Institute of Science and Technology (KIST), Gangneung, 25451, Republic of Korea.
³ Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea.
⁴ Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea.
⁵ Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon National University, Chunchon, 24341, Republic of Korea.
⁶ Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea. Electronic address: [email protected].
⁷ Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea. Electronic address: [email protected].

PMID: 27686478
DOI: 10.1016/j.virusres.2016.09.013

Abstract

A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH₃OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40μM.

Keywords: Antiviral agent; Brucea javanica; Capsicum annuum; Green fluorescent protein; Pepper mottle virus; Quassinoid.

MeSH terms

Antiviral Agents / chemistry
Antiviral Agents / pharmacology*
Brucea / chemistry*
Microbial Sensitivity Tests
Piperaceae / virology*
Plant Extracts / pharmacology*
Potyvirus / drug effects*
Potyvirus / physiology*
Quassins / chemistry
Quassins / pharmacology*

Substances

Antiviral Agents
Plant Extracts
Quassins

Supplementary concepts

Pepper mottle virus