Evaluation of the safety, pharmacokinetics, pharmacodynamics, and drug-drug interaction potential of a selective Lp-PLA2 inhibitor (GSK2647544) in healthy volunteers

Int J Clin Pharmacol Ther. 2016 Dec;54(12):935-949. doi: 10.5414/CP202565.

Abstract

Objective: To evaluate in healthy volunteers the safety, pharmacokinetics (PK), pharmacodynamics (PD), and drug-drug interaction (DDI) potential of GSK2647544, (a selective lipoprotein-associated phospholipase A2 (Lp-PLA2) inhibitor).

Methods: Study 1 was a single-blind, randomized, placebo-controlled, crossover study with healthy male volunteers randomized to receive single escalating oral doses (0.5 - 750 mg) of GSK2647544. Study 2 was a single-blind, randomized, placebo-controlled study with healthy volunteers randomized to receive repeat doses (80 mg) of GSK2647544. The drug-drug interaction of GSK2647544 with simvastatin was also evaluated in study 2.

Results: Across both studies GSK2647544 doses were generally well tolerated with no GSK2647544-related clinically significant findings. GSK2647544 was readily absorbed and its plasma concentration declined bi-exponentially with a terminal half-life ranging from 8 to 16 hours. Plasma exposure of GSK2647544 increased approximately dose-proportionally. There was GSK2647544 dose-dependent inhibition of plasma Lp-PLA2 activity, with a trough inhibition (12 hours after dose) of 85.6% after 7-day twice daily dosing. The administration of simvastatin concomitantly with GSK2647544 increased the overall exposure (area under the plasma concentration-time curve and maximum plasma concentration) of simvastatin and simvastatin acid by 3.6- to 4.3-fold and 1.5- to 3.1-fold, respectively.

Conclusions: GSK2647544 was generally well tolerated and had a reasonable PK-PD profile. The clinically significant drug-drug interaction led to an early termination of study 2. .

Publication types

  • Randomized Controlled Trial

MeSH terms

  • 1-Alkyl-2-acetylglycerophosphocholine Esterase / antagonists & inhibitors*
  • Adult
  • Cytochrome P-450 CYP3A Inhibitors / pharmacology
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Female
  • Healthy Volunteers
  • Humans
  • Male
  • Phenyl Ethers / adverse effects*
  • Phenyl Ethers / pharmacokinetics
  • Phenyl Ethers / pharmacology
  • Pyrimidinones / adverse effects*
  • Pyrimidinones / pharmacokinetics
  • Pyrimidinones / pharmacology
  • Simvastatin / pharmacology
  • Single-Blind Method

Substances

  • Cytochrome P-450 CYP3A Inhibitors
  • GSK2647544
  • Phenyl Ethers
  • Pyrimidinones
  • Simvastatin
  • 1-Alkyl-2-acetylglycerophosphocholine Esterase