Studies of G-quadruplexes formed within self-assembled DNA mini-circles

Chem Commun (Camb). 2016 Oct 13;52(84):12454-12457. doi: 10.1039/c6cc07110d.

Abstract

We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.

MeSH terms

  • DNA, Circular / chemistry*
  • G-Quadruplexes*
  • Humans
  • Kinetics
  • Promoter Regions, Genetic / genetics*
  • Proto-Oncogene Proteins c-myc / genetics*
  • Thermodynamics

Substances

  • DNA, Circular
  • Proto-Oncogene Proteins c-myc