Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6

Kaifeng Wang; Yue Wu; Dan Yin; Shixing Tang; Guifang Hu; Yaqing He

doi:10.1007/s00705-016-3100-8

Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6

Arch Virol. 2017 Jan;162(1):287-290. doi: 10.1007/s00705-016-3100-8. Epub 2016 Oct 8.

Authors

Kaifeng Wang¹, Yue Wu¹, Dan Yin¹, Shixing Tang¹, Guifang Hu², Yaqing He³

Affiliations

¹ Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China.
² Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, 510515, China. [email protected].
³ Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, Guangdong, China. [email protected].

PMID: 27722991
DOI: 10.1007/s00705-016-3100-8

Abstract

Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.

Keywords: Coxsackievirus A6; Hand, foot and mouth disease; Recombinase polymerase amplification.

Publication types

Evaluation Study

MeSH terms

Child, Preschool
Enterovirus / genetics
Enterovirus / isolation & purification*
Hand, Foot and Mouth Disease / diagnosis*
Humans
Infant
Infant, Newborn
Nucleic Acid Amplification Techniques / methods*
Real-Time Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Time Factors