AuNP-Collagen Matrix with Localized Stiffness for Cardiac-Tissue Engineering: Enhancing the Assembly of Intercalated Discs by β1-Integrin-Mediated Signaling

Adv Mater. 2016 Dec;28(46):10230-10235. doi: 10.1002/adma.201603027. Epub 2016 Oct 10.

Abstract

A schematic for the mechanism of accelerating the assembly of intercalated discs (IDs) in cardiac myocytes regulated by gold nanoparticles (AuNPs) is presented. AuNPs with local nanoscale stiffness in the substrate activate β1-integrin signaling, which mediates the activation of integrin-linked kinase (ILK) and its downstream signal kinase by stimulating expression of the transcription factors GATA4 and MEF-2c.

Keywords: cardiac-tissue engineering; gold nanoparticles; intercalated discs; localized stiffness; β1-integrin.

MeSH terms

  • Animals
  • Cells, Cultured
  • Collagen / metabolism*
  • GATA4 Transcription Factor / metabolism
  • Gold / chemistry*
  • Integrin beta1 / metabolism*
  • MEF2 Transcription Factors / metabolism
  • Metal Nanoparticles / chemistry*
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / metabolism
  • Protein Serine-Threonine Kinases / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction
  • Tissue Engineering / methods*
  • Tissue Scaffolds / chemistry*

Substances

  • GATA4 Transcription Factor
  • Gata4 protein, rat
  • Integrin beta1
  • MEF2 Transcription Factors
  • MEF2C protein, rat
  • Gold
  • Collagen
  • integrin-linked kinase
  • Protein Serine-Threonine Kinases