Abstract
Binary expression systems are flexible and versatile genetic tools in Drosophila. The Q-system is a recently developed repressible binary expression system that offers new possibilities for transgene expression and genetic manipulations. In this review chapter, we focus on current state-of-the-art Q-system tools and reagents. We also discuss in vivo applications of the Q-system, together with GAL4/UAS and LexA/LexAop systems, for simultaneous expression of multiple effectors, intersectional labeling, and clonal analysis.
Keywords:
Binary expression system; Chimeric transactivators; GAL4QF; Intersectional expression; LexAQF; MARCM; Mitotic recombination; Mosaic analysis; Neurospora crassa; Q-system; QF; QF2; QF2w; QS; QUAS; Quinic acid.
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Drosophila Proteins / antagonists & inhibitors
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / metabolism
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Gene Expression Profiling
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Gene Expression Regulation*
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Genes, Reporter
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Genetic Techniques
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Genetic Vectors / chemistry
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Genetic Vectors / metabolism*
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Neurospora crassa / genetics*
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Neurospora crassa / metabolism
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Serine Endopeptidases / genetics
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Serine Endopeptidases / metabolism
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Signal Transduction
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Transcription Factors / antagonists & inhibitors
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcription, Genetic
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Transgenes*
Substances
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Bacterial Proteins
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Drosophila Proteins
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GAL4 protein, Drosophila
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GAL80 protein, S cerevisiae
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LexA protein, Bacteria
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RNA, Small Interfering
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Serine Endopeptidases