Characterisation of siRNAs derived from new isolates of bamboo mosaic virus and their associated satellites in infected ma bamboo (Dendrocalamus latiflorus)

Arch Virol. 2017 Feb;162(2):505-510. doi: 10.1007/s00705-016-3092-4. Epub 2016 Oct 14.

Abstract

We characterised the virus-derived small interfering RNAs (vsiRNA) of bamboo mosaic virus (Ba-vsiRNAs) and its associated satellite RNA (satRNA)-derived siRNAs (satsiRNAs) in a bamboo plant (Dendrocalamus latiflorus) by deep sequencing. Ba-vsiRNAs and satsiRNAs of 21-22 nt in length, with both (+) and (-) polarity, predominated. The 5'-terminal base of Ba-vsiRNA was biased towards A, whereas a bias towards C/U was observed in sense satsiRNAs, and towards A in antisense satsiRNAs. A large set of bamboo genes were identified as potential targets of Ba-vsiRNAs and satsiRNAs, revealing RNA silencing-based virus-host interactions in plants. Moreover, we isolated and characterised new isolates of bamboo mosaic virus (BaMV; 6,350 nt) and BaMV-associated satRNA (satBaMV; 834 nt), designated BaMV-MAZSL1 and satBaMV-MAZSL1, respectively.

MeSH terms

  • Bambusa / virology*
  • Genes, Plant*
  • High-Throughput Nucleotide Sequencing
  • Host-Pathogen Interactions
  • Open Reading Frames
  • Plant Diseases / virology
  • Potexvirus / classification
  • Potexvirus / genetics*
  • Potexvirus / isolation & purification
  • RNA Interference
  • RNA, Satellite / genetics*
  • RNA, Small Interfering / genetics*
  • RNA, Viral / genetics*

Substances

  • RNA, Satellite
  • RNA, Small Interfering
  • RNA, Viral