Abstract
Bovine ephemeral fever (BEF) is caused by the arthropod-borne bovine ephemeral fever virus (BEFV), which is a member of the family Rhabdoviridae and the genus Ephemerovirus. BEFV causes an acute febrile infection in cattle and water buffalo. In this study, a recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of BEFV (rL-BEFV-G) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. BEFV G enabled NDV to spread from cell to cell. rL-BEFV-G remained nonvirulent in poultry and mice compared with vector LaSota virus. rL-BEFV-G triggered a high titer of neutralizing antibodies against BEFV in mice and cattle. These results suggest that rL-BEFV-G might be a suitable candidate vaccine against BEF.
MeSH terms
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Animals
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Antibodies, Neutralizing / biosynthesis*
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Antibodies, Viral / biosynthesis*
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Cattle
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Chick Embryo
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Cricetinae
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Dogs
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Ephemeral Fever / immunology
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Ephemeral Fever / prevention & control*
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Ephemeral Fever / virology
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Ephemeral Fever Virus, Bovine / drug effects
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Ephemeral Fever Virus, Bovine / genetics*
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Ephemeral Fever Virus, Bovine / immunology
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Epithelial Cells / virology
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Gene Expression
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Genetic Vectors / chemistry
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Genetic Vectors / immunology
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Glycoproteins / administration & dosage
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Glycoproteins / genetics
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Glycoproteins / immunology
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Immunization
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Madin Darby Canine Kidney Cells
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Mice
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Mice, Inbred BALB C
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Newcastle disease virus / genetics*
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Newcastle disease virus / immunology
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Reassortant Viruses / genetics
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Reassortant Viruses / immunology
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Vaccines, Synthetic
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Viral Proteins / administration & dosage
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Viral Proteins / genetics
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Viral Proteins / immunology
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Viral Vaccines / administration & dosage
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Viral Vaccines / genetics*
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Viral Vaccines / immunology
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Glycoproteins
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Vaccines, Synthetic
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Viral Proteins
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Viral Vaccines