Activity of c-Met/ALK Inhibitor Crizotinib and Multi-Kinase VEGF Inhibitor Pazopanib in Metastatic Gastrointestinal Neuroectodermal Tumor Harboring EWSR1-CREB1 Fusion

Oncology. 2016;91(6):348-353. doi: 10.1159/000449204. Epub 2016 Oct 21.

Abstract

Malignant gastrointestinal neuroectodermal tumor (GNET) is an aggressive rare tumor, primarily occurring in young adults with frequent local-regional metastases and recurrence after local control. The tumor is characterized by the presence of EWSR1-ATF1 or EWSR1-CREB1 and immunohistochemical positivity for S-100 protein without melanocytic marker positivity. Due to poor responses to standard sarcoma regimens, GNET has a poor prognosis, and development of effective systemic therapy is desperately needed to treat these patients. Herein, we present a patient with a small bowel GNET who experienced recurrent hepatic and skeletal metastases after a primary resection. Comprehensive genomic profiling (CGP) in the course of clinical care with DNA and RNA sequencing demonstrated the presence of an exon 7 to exon 6 EWSR1-CREB1 fusion in the context of a diploid genome with no other genomic alterations. In a clinical trial, the patient received a combination of 250 mg crizotinib with 600 mg pazopanib quaque die and achieved partial response and durable clinical benefit for over 2.8 years, and with minimal toxicity from therapy. Using a CGP database of over 50,000 samples, we identified 11 additional cases that harbor EWSR1-CREB1 and report clinicopathologic characteristics, as these patients may also benefit from such a regimen.

Publication types

  • Case Reports

MeSH terms

  • Adolescent
  • Adult
  • Anaplastic Lymphoma Kinase
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
  • Bone Neoplasms / drug therapy*
  • Bone Neoplasms / secondary
  • Crizotinib
  • Female
  • Humans
  • Indazoles
  • Intestinal Neoplasms / drug therapy*
  • Intestinal Neoplasms / genetics*
  • Intestinal Neoplasms / pathology
  • Liver Neoplasms / drug therapy*
  • Liver Neoplasms / secondary
  • Male
  • Middle Aged
  • Neuroectodermal Tumors / drug therapy*
  • Neuroectodermal Tumors / genetics*
  • Neuroectodermal Tumors / secondary
  • Oncogene Proteins, Fusion / genetics*
  • Proto-Oncogene Proteins c-met / antagonists & inhibitors
  • Pyrazoles / administration & dosage
  • Pyridines / administration & dosage
  • Pyrimidines / administration & dosage
  • Receptor Protein-Tyrosine Kinases / antagonists & inhibitors
  • Response Evaluation Criteria in Solid Tumors
  • Sulfonamides / administration & dosage
  • Vascular Endothelial Growth Factor A / antagonists & inhibitors
  • Young Adult

Substances

  • EWSR1-CREB1 fusion protein, human
  • Indazoles
  • Oncogene Proteins, Fusion
  • Pyrazoles
  • Pyridines
  • Pyrimidines
  • Sulfonamides
  • Vascular Endothelial Growth Factor A
  • Crizotinib
  • pazopanib
  • ALK protein, human
  • Anaplastic Lymphoma Kinase
  • Proto-Oncogene Proteins c-met
  • Receptor Protein-Tyrosine Kinases