CD4+ Foxp3+ T cells promote aberrant immunoglobulin G production and maintain CD8+ T-cell suppression during chronic liver disease

Hepatology. 2017 Feb;65(2):661-677. doi: 10.1002/hep.28894. Epub 2016 Dec 19.

Abstract

Persistent hepatotropic viral infections are a common etiologic agent of chronic liver disease. Unresolved infection can be attributed to nonfunctional intrahepatic CD8+ T-cell responses. In light of dampened CD8+ T-cell responses, liver disease often manifests systemically as immunoglobulin (Ig)-related syndromes due to aberrant B-cell functions. These two opposing yet coexisting phenomena implicate the potential of altered CD4+ T-cell help. Elevated CD4+ forkhead box P3-positive (Foxp3+) T cells were evident in both human liver disease and a mouse model of chemically induced liver injury despite marked activation and spontaneous IgG production by intrahepatic B cells. While this population suppressed CD8+ T-cell responses, aberrant B-cell activities were maintained due to expression of CD40 ligand on a subset of CD4+ Foxp3+ T cells. In vivo blockade of CD40 ligand attenuated B-cell abnormalities in a mouse model of liver injury. A phenotypically similar population of CD4+ Foxp3+, CD40 ligand-positive T cells was found in diseased livers explanted from patients with chronic hepatitis C infection. This population was absent in nondiseased liver tissues and peripheral blood.

Conclusion: Liver disease elicits alterations in the intrahepatic CD4+ T-cell compartment that suppress T-cell immunity while concomitantly promoting aberrant IgG mediated manifestations. (Hepatology 2017;65:661-677).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • Cells, Cultured
  • Chronic Disease
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Enzyme-Linked Immunospot Assay
  • Flow Cytometry
  • Forkhead Transcription Factors / metabolism*
  • Hepatitis C, Chronic / immunology
  • Hepatitis C, Chronic / pathology
  • Hepatocytes
  • Humans
  • Immunoglobulin G / immunology*
  • Immunoglobulin G / metabolism
  • Immunohistochemistry
  • Liver Cirrhosis / immunology*
  • Liver Cirrhosis / pathology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Random Allocation
  • Statistics, Nonparametric
  • T-Lymphocytes, Regulatory / immunology

Substances

  • Forkhead Transcription Factors
  • Immunoglobulin G