Lectin adhesion proteins (P-, L- and E-selectins) as biomarkers in colorectal cancer

Biomarkers. 2017 Nov;22(7):629-634. doi: 10.1080/1354750X.2016.1252967. Epub 2016 Nov 9.

Abstract

Context: Selectins probably participate in the interactions between platelets and other inflammatory cells in cancer invasion and metastasis formation. We assessed a potential relationship of P-, L- and E-selectin in colorectal cancer (CRC) patients in relation to tumour advancement according to TNM classification, and tumour location.

Materials and methods: The study group was composed of 53 CRC patients and 25 healthy subjects. Plasma levels of soluble P-, L- and E-selectins were measured using the immunoenzymatic method with Quantikine kits (R&D Systems, Minneapolis, MN).

Results: The mean levels of all selectins were significantly higher in CRC patients compared to healthy controls. The highest level of sP-selectin was observed in patients with metastases to the liver (stage IV), and was significantly higher than in patients without metastases (stage I/II) and with lymph node metastases (stage III), p = .02. The highest levels of sL- and sE-selectin were observed in patients with lymph node metastasis. We also found sP-selectin to be the best predictor of CRC.

Conclusion: Our finding show possible involvement of tested selectins in CRC advancement and forming metastasis. Among sL- and E- selectins, P-selectin plays an important role in the progression of CRC and could be an attractive biomarker with clinical significance.

Keywords: Colorectal cancer; biomarker; metastasis; sE-selectin; sL-selectin; sP-selectin.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / blood*
  • Case-Control Studies
  • Cell Adhesion Molecules / blood*
  • Colorectal Neoplasms / diagnosis
  • Colorectal Neoplasms / pathology*
  • E-Selectin
  • Female
  • Humans
  • L-Selectin
  • Liver Neoplasms / secondary
  • Male
  • Middle Aged
  • Neoplasm Metastasis / diagnosis
  • P-Selectin / blood*

Substances

  • Biomarkers, Tumor
  • Cell Adhesion Molecules
  • E-Selectin
  • P-Selectin
  • SELE protein, human
  • SELL protein, human
  • SELP protein, human
  • L-Selectin