Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions

Malin Kele; Kelly Day; Harriet Rönnholm; Jens Schuster; Niklas Dahl; Anna Falk

doi:10.1016/j.scr.2016.09.028

Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions

Stem Cell Res. 2016 Nov;17(3):474-478. doi: 10.1016/j.scr.2016.09.028. Epub 2016 Sep 30.

Authors

Malin Kele¹, Kelly Day¹, Harriet Rönnholm¹, Jens Schuster², Niklas Dahl², Anna Falk³

Affiliations

¹ Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden.
² Uppsala University, Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Box 815, 751 08 Uppsala, Sweden.
³ Karolinska Institutet, Department of Neuroscience, Retziusvag 8, 171 77 Stockholm, Sweden. Electronic address: [email protected].

PMID: 27789397
DOI: 10.1016/j.scr.2016.09.028

Abstract

CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.

MeSH terms

Cell Differentiation
Cell Line
Cellular Reprogramming
Culture Media / chemistry*
Embryoid Bodies / cytology
Embryoid Bodies / metabolism
Fibroblasts / cytology*
Genetic Vectors / genetics
Genetic Vectors / metabolism
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Karyotype
Kruppel-Like Factor 4
Male
Microscopy, Fluorescence
Sendai virus / genetics
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Culture Media
KLF4 protein, human
Kruppel-Like Factor 4
Transcription Factors