Weakly self-reactive T-cell clones can homeostatically expand when present at low numbers

Eur J Immunol. 2017 Jan;47(1):68-73. doi: 10.1002/eji.201646540. Epub 2016 Nov 18.

Abstract

T-cell division is central to maintaining a stable T-cell pool in adults. It also enables T-cell expansion in neonates, and after depletion by chemotherapy, bone marrow transplantation, or infection. The same signals required for T-cell survival in lymphoreplete settings, IL-7 and T-cell receptor (TCR) interactions with self-peptide MHC (pMHC), induce division when T-cell numbers are low. The strength of reactivity for self-pMHC has been shown to correlate with the capacity of T cells to undergo lymphopenia-induced proliferation (LIP), in that weakly self-reactive T cells are unable to divide, implying that T-cell reconstitution would significantly skew the TCR repertoire toward TCRs with greater self-reactivity and thus compromise T-cell diversity. Here, we show that while CD4+ T cells with low self-pMHC reactivity experience more intense competition, they are able to divide when present at low enough cell numbers. Thus, at physiological precursor frequencies CD4+ T cells with low self-pMHC reactivity are able to contribute to the reconstitution of the T-cell pool.

Keywords: Lymphopenia-induced proliferation; Regulation of homeostasis; T-cell competition; T-cell diversity; T-cell repertoire.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Autoimmunity*
  • Biomarkers
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • Cell Proliferation
  • Clone Cells
  • Gene Expression
  • Homeostasis
  • Immunophenotyping
  • Lymphocyte Activation / immunology
  • Lymphocyte Count
  • Lymphopenia / immunology
  • Major Histocompatibility Complex / immunology
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Phenotype
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / metabolism
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism

Substances

  • Antigens, CD
  • Biomarkers
  • Receptors, Antigen, T-Cell