Abstract
Gastrointestinal stromal tumors (GIST) arise within the interstitial cell of Cajal (ICC) lineage due to activating KIT/PDGFRA mutations. Both ICC and GIST possess primary cilia (PC), which coordinate PDGFRA and Hedgehog signaling, regulators of gastrointestinal mesenchymal development. Therefore, we hypothesized that Hedgehog signaling may be altered in human GIST and controls KIT expression. Quantitative RT-PCR, microarrays, and next generation sequencing were used to describe Hedgehog/PC-related genes in purified human ICC and GIST. Genetic and pharmacologic approaches were employed to investigate the effects of GLI manipulation on KIT expression and GIST cell viability. We report that Hedgehog pathway and PC components are expressed in ICC and GIST and subject to dysregulation during GIST oncogenesis, irrespective of KIT/PDGFRA mutation status. Using genomic profiling, 10.2% of 186 GIST studied had potentially deleterious genomic alterations in 5 Hedgehog-related genes analyzed, including in the PTCH1 tumor suppressor (1.6%). Expression of the predominantly repressive GLI isoform, GLI3, was inversely correlated with KIT mRNA levels in GIST cells and non-KIT/non-PDGFRA mutant GIST. Overexpression of the 83-kDa repressive form of GLI3 or small interfering RNA-mediated knockdown of the activating isoforms GLI1/2 reduced KIT mRNA. Treatment with GLI1/2 inhibitors, including arsenic trioxide, significantly increased GLI3 binding to the KIT promoter, decreased KIT expression, and reduced viability in imatinib-sensitive and imatinib-resistant GIST cells. These data offer new evidence that genes necessary for Hedgehog signaling and PC function in ICC are dysregulated in GIST. Hedgehog signaling activates KIT expression irrespective of mutation status, offering a novel approach to treat imatinib-resistant GIST.
Keywords:
GIST; GLI; ICC; arsenic trioxide; imatinib-resistant.
MeSH terms
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Antineoplastic Agents / pharmacology
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Arsenic Trioxide
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Arsenicals / pharmacology
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Binding Sites
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Cell Line, Tumor
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Cell Survival
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Cilia / metabolism
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Cilia / pathology
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Dose-Response Relationship, Drug
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Drug Resistance, Neoplasm
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Gastrointestinal Neoplasms / drug therapy
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Gastrointestinal Neoplasms / genetics
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Gastrointestinal Neoplasms / metabolism*
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Gastrointestinal Neoplasms / pathology
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Gastrointestinal Stromal Tumors / drug therapy
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Gastrointestinal Stromal Tumors / genetics
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Gastrointestinal Stromal Tumors / metabolism*
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Gastrointestinal Stromal Tumors / pathology
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Gene Expression Regulation, Neoplastic
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Humans
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Imatinib Mesylate / pharmacology
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Interstitial Cells of Cajal / drug effects
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Interstitial Cells of Cajal / metabolism*
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Interstitial Cells of Cajal / pathology
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Mutation
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Nuclear Proteins / antagonists & inhibitors
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Oxides / pharmacology
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Promoter Regions, Genetic
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Proto-Oncogene Proteins c-kit / genetics
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Proto-Oncogene Proteins c-kit / metabolism*
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RNA Interference
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptor, Platelet-Derived Growth Factor alpha / genetics
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Signal Transduction* / drug effects
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Transfection
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Zinc Finger Protein GLI1 / antagonists & inhibitors
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Zinc Finger Protein GLI1 / genetics
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Zinc Finger Protein GLI1 / metabolism*
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Zinc Finger Protein Gli2 / antagonists & inhibitors
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Zinc Finger Protein Gli2 / genetics
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Zinc Finger Protein Gli2 / metabolism*
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Zinc Finger Protein Gli3 / genetics
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Zinc Finger Protein Gli3 / metabolism*
Substances
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Antineoplastic Agents
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Arsenicals
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GLI1 protein, human
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GLI2 protein, human
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GLI3 protein, human
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Nerve Tissue Proteins
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Nuclear Proteins
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Oxides
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RNA, Messenger
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Zinc Finger Protein GLI1
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Zinc Finger Protein Gli2
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Zinc Finger Protein Gli3
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Imatinib Mesylate
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Proto-Oncogene Proteins c-kit
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Receptor, Platelet-Derived Growth Factor alpha
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Arsenic Trioxide