Evaluation of the Carba NP Test in Oregon, 2013

Antimicrob Agents Chemother. 2016 Dec 27;61(1):e03005-15. doi: 10.1128/AAC.03005-15. Print 2017 Jan.

Abstract

Carbapenem-resistant Enterobacteriaceae (CRE) are an urgent public health threat. We evaluated the capacity of the Carba NP test to detect carbapenemase production in 206 isolates: 143 Enterobacteriaceae identified by Oregon's CRE surveillance program in 2013 and 63 known carbapenemase-positive organisms. Overall, test sensitivity and specificity were 89% (59/66 isolates; 95% confidence interval [CI], 81 to 97%) and 100% (140/140 isolates; 95% CI, 98 to 100%), respectively. All KPC, NDM-1, VIM, and IMP producers but no (0/7 isolates) OXA-48-like strains were Carba NP positive prior to a post hoc protocol modification. We subsequently incorporated Carba NP into Oregon's CRE screening algorithm.

Keywords: Carba NP; IMP; KPC; NDM; OXA-48; VIM; carbapenemase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / classification
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Carbapenems / pharmacology*
  • Enterobacteriaceae / drug effects*
  • Enterobacteriaceae / growth & development
  • Enterobacteriaceae / pathogenicity
  • Enterobacteriaceae Infections / drug therapy
  • Enterobacteriaceae Infections / microbiology
  • Gene Expression
  • Humans
  • Microbial Sensitivity Tests
  • Sensitivity and Specificity
  • beta-Lactamases / classification
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • beta-Lactamases
  • carbapenemase