miR-374 promotes myocardial hypertrophy by negatively regulating vascular endothelial growth factor receptor-1 signaling

Jong Sub Lee; Dong Woo Song; Jei Hyoung Park; Jin Ock Kim; Chunghee Cho; Do Han Kim

doi:10.5483/bmbrep.2017.50.4.165

miR-374 promotes myocardial hypertrophy by negatively regulating vascular endothelial growth factor receptor-1 signaling

BMB Rep. 2017 Apr;50(4):208-213. doi: 10.5483/bmbrep.2017.50.4.165.

Authors

Jong Sub Lee¹, Dong Woo Song¹, Jei Hyoung Park¹, Jin Ock Kim¹, Chunghee Cho¹, Do Han Kim¹

Affiliation

¹ School of Life Sciences and Systems Biology Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea.

Abstract

Vascular endothelial growth factor (VEGF) is an essential cytokine that has functions in the formation of new blood vessels and regression of cardiac hypertrophy. VEGF/VEGF-receptor-1 (VEGFR1) signaling plays a key role in the regression of cardiac hypertrophy, whereas VEGF/VEGFR2 signaling leads to cardiac hypertrophy. In this study, we identified the prohypertrophic role of miR-374 using neonatal rat ventricular myocytes (NRVMs). Our results showed that overexpression of miR-374 activated G protein-coupled receptor-mediated prohypertrophic pathways by the inhibition of VEGFR1-dependent regression pathways. Luciferase assays revealed that miR-374 could directly target the 3'-untranslated regions of VEGFR1 and cGMP-dependent protein kinase-1. Collectively, these findings demonstrated that miR-374 was a novel pro-hypertrophic microRNA functioning to suppress the VEGFR1-mediated regression pathway. [BMB Reports 2017; 50(4): 208-213].

MeSH terms

3' Untranslated Regions
Animals
Antagomirs / metabolism
Base Sequence
Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism
Cardiomegaly / genetics
Cardiomegaly / metabolism
Cardiomegaly / pathology
Cells, Cultured
Cyclic GMP-Dependent Protein Kinase Type I / antagonists & inhibitors
Cyclic GMP-Dependent Protein Kinase Type I / genetics
Cyclic GMP-Dependent Protein Kinase Type I / metabolism
Genes, Reporter
MEF2 Transcription Factors / metabolism
MicroRNAs / antagonists & inhibitors
MicroRNAs / genetics
MicroRNAs / metabolism*
Myocytes, Cardiac / cytology
Myocytes, Cardiac / metabolism
NFATC Transcription Factors / metabolism
RNA Interference
RNA, Small Interfering / metabolism
Rats
Rats, Sprague-Dawley
Sequence Alignment
Signal Transduction*
Vascular Endothelial Growth Factor Receptor-1 / antagonists & inhibitors
Vascular Endothelial Growth Factor Receptor-1 / genetics
Vascular Endothelial Growth Factor Receptor-1 / metabolism*

Substances

3' Untranslated Regions
Antagomirs
MEF2 Transcription Factors
MicroRNAs
NFATC Transcription Factors
RNA, Small Interfering
Vascular Endothelial Growth Factor Receptor-1
Cyclic GMP-Dependent Protein Kinase Type I
Calcium-Calmodulin-Dependent Protein Kinase Type 2