Effects of Structural Variations on the Cellular Response and Mechanical Properties of Biocompatible, Biodegradable, and Porous Smectic Liquid Crystal Elastomers

Macromol Biosci. 2017 Feb;17(2). doi: 10.1002/mabi.201600278. Epub 2016 Nov 2.

Abstract

The authors report on series of side-chain smectic liquid crystal elastomer (LCE) cell scaffolds based on star block-copolymers featuring 3-arm, 4-arm, and 6-arm central nodes. A particular focus of these studies is placed on the mechanical properties of these LCEs and their impact on cell response. The introduction of diverse central nodes allows to alter and custom-modify the mechanical properties of LCE scaffolds to values on the same order of magnitude of various tissues of interest. In addition, it is continued to vary the position of the LC pendant group. The central node and the position of cholesterol pendants in the backbone of ε-CL blocks (alpha and gamma series) affect the mechanical properties as well as cell proliferation and particularly cell alignment. Cell directionality tests are presented demonstrating that several LCE scaffolds show cell attachment, proliferation, narrow orientational dispersion of cells, and highly anisotropic cell growth on the as-synthesized LCE materials.

Keywords: 3D porous scaffolds; cell alignment; cell directionality; liquid crystal elastomers; mechanics.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials / chemical synthesis
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology
  • Cell Line
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Dermis / cytology
  • Elastomers / chemical synthesis
  • Elastomers / chemistry*
  • Elastomers / pharmacology
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Humans
  • Liquid Crystals / chemistry*
  • Liquid Crystals / ultrastructure
  • Mechanical Phenomena*
  • Mice
  • Microscopy, Polarization
  • Myoblasts / cytology
  • Myoblasts / drug effects
  • Porosity
  • Scattering, Small Angle
  • Stress, Mechanical
  • Temperature
  • X-Ray Diffraction

Substances

  • Biocompatible Materials
  • Elastomers