Lymphoepithelioma-like carcinoma of breast-evaluation for Epstein-Barr virus-encoded RNA, human papillomavirus, and markers of basal cell differentiation

Ann Diagn Pathol. 2016 Dec:25:42-47. doi: 10.1016/j.anndiagpath.2016.05.006. Epub 2016 Aug 16.

Abstract

This is a largest series of 5 cases of lymphoepithelioma-like carcinoma (LEC) of the breast attempting to look at the expression of basal cytokeratins (CKs), human papillomavirus, and Epstein-Barr virus-encoded RNAs in these tumors. Five cases were selected after stringent evaluation of all breast carcinomas showing dense lymphoid infiltration. Histologically, all these tumors showed the typical histology except 1 tumor that showed an unusual granulomatous response. All tumors were negative for estrogen and progesterone receptors and HER2 (triple negative). Three tumors expressed CK5/6 and high-molecular-weight CK, whereas only the case with nodal metastasis expressed CK14. Analysis for in situ hybridization for Epstein-Barr virus-encoded RNAs and human papillomavirus DNA on paraffin-processed tissues was negative in all tumors. All of these patients received adjuvant therapy. One patient with tumor expressing basal marker, CK5/6, had contralateral breast malignancy after a duration of 53 months of treatment completion. The rest were disease free with the follow-up period in the range of 6 to 105 months. The lymphoepithelioma-like carcinoma of breast expressed basal CK profile that is more CK5/6 positive than CK14. Analysis of basal markers within these tumors may help in refining the definition of these tumors and in classifying them into prognostically relevant groups.

Keywords: Basal cytokeratins; Breast; EBER-ISH; HPV; Lymphoepithelioma-like carcinoma.

MeSH terms

  • Adult
  • Aged
  • Breast Neoplasms / diagnosis*
  • Breast Neoplasms / pathology
  • Breast Neoplasms / virology*
  • Carcinoma, Squamous Cell / pathology*
  • Carcinoma, Squamous Cell / virology
  • Cell Differentiation / physiology
  • DNA, Viral / analysis*
  • Female
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Immunohistochemistry / methods
  • In Situ Hybridization / methods
  • Middle Aged
  • Papillomaviridae / isolation & purification*
  • Prognosis
  • Receptors, Progesterone / genetics

Substances

  • DNA, Viral
  • Receptors, Progesterone