Archetype and Rearranged Non-coding Control Regions in Urothelial Bladder Carcinoma of Immunocompetent Individuals

Cancer Genomics Proteomics. 2016;13(6):499-509. doi: 10.21873/cgp.20013.

Abstract

Background: Polyomaviruses (PyVs) are potential transforming viruses. Despite their involvement in human tumours still being debated, there is evidence to suggest a role for PyVs in bladder carcinoma (BC). Therefore, a possible association between PyVs and BC was investigated.

Materials and methods: Urine, blood and fresh bladder tissue specimens were collected from 29 patients with BC. PyV prevalence, non-coding control region (NCCR) organization and genotypic analysis were assessed.

Results: Data showed a significant prevalence of John Cunningham (JC) PyV in BC tissues and in urine with respect to BKPyV, while simian virus 40 was not revealed. A BKPyV rearranged NCCR sequence was isolated, whereas a JCPyV archetypal structure was consistently retained. A prevalence of European genotypes was observed.

Conclusion: Our data would suggest a JCPyV involvement in cancer progression and a BKPyV association with BC pathogenesis in immunocompetent patients. However, further work is necessary to better understand the exact role of PyVs in urothelial carcinogenesis.

Keywords: BKPyV; Polyomaviruses; PyVs; urothelial bladder carcinoma.

MeSH terms

  • Aged
  • Aged, 80 and over
  • BK Virus / genetics*
  • BK Virus / isolation & purification
  • BK Virus / pathogenicity
  • Female
  • Gene Rearrangement
  • Genotype
  • Humans
  • JC Virus / genetics*
  • JC Virus / isolation & purification
  • JC Virus / pathogenicity
  • Male
  • Middle Aged
  • RNA, Untranslated / genetics*
  • Urinary Bladder Neoplasms / blood
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / urine
  • Urinary Bladder Neoplasms / virology
  • Urothelium / pathology
  • Urothelium / virology

Substances

  • RNA, Untranslated