Revealing the vectors of cellular identity with single-cell genomics

Nat Biotechnol. 2016 Nov 8;34(11):1145-1160. doi: 10.1038/nbt.3711.

Abstract

Single-cell genomics has now made it possible to create a comprehensive atlas of human cells. At the same time, it has reopened definitions of a cell's identity and of the ways in which identity is regulated by the cell's molecular circuitry. Emerging computational analysis methods, especially in single-cell RNA sequencing (scRNA-seq), have already begun to reveal, in a data-driven way, the diverse simultaneous facets of a cell's identity, from discrete cell types to continuous dynamic transitions and spatial locations. These developments will eventually allow a cell to be represented as a superposition of 'basis vectors', each determining a different (but possibly dependent) aspect of cellular organization and function. However, computational methods must also overcome considerable challenges-from handling technical noise and data scale to forming new abstractions of biology. As the scale of single-cell experiments continues to increase, new computational approaches will be essential for constructing and characterizing a reference map of cell identities.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Physiological Phenomena / physiology*
  • Computer Simulation
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation / physiology*
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Models, Biological
  • Proteome / metabolism*
  • Signal Transduction / physiology
  • Transcriptome / physiology*

Substances

  • Proteome