Enhanced electrogenerated chemiluminescence behavior of C3N4 QDs@ C3N4 nanosheet and its signal-on aptasensing for platelet derived growth factor

Huifeng Xu; Shijing Liang; Xi Zhu; Xiuqin Wu; Yongqiang Dong; Haishan Wu; Wenxia Zhang; Yuwu Chi

doi:10.1016/j.bios.2016.10.026

Enhanced electrogenerated chemiluminescence behavior of C₃N₄ QDs@ C₃N₄ nanosheet and its signal-on aptasensing for platelet derived growth factor

Biosens Bioelectron. 2017 Jun 15:92:695-701. doi: 10.1016/j.bios.2016.10.026. Epub 2016 Oct 14.

Authors

Huifeng Xu¹, Shijing Liang², Xi Zhu³, Xiuqin Wu², Yongqiang Dong⁴, Haishan Wu⁴, Wenxia Zhang⁵, Yuwu Chi⁶

Affiliations

¹ MOE Key Laboratory of Analysis and Detection for Food Safety, State Key Laboratory of Photo catalysis on Energy and Environment, and College of Chemistry, Fuzhou University, Fujian 350108, PR China; Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China.
² Department of Environmental Science and Engineering, Fuzhou University, Minhou, Fujian 350108, PR China.
³ College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
⁴ MOE Key Laboratory of Analysis and Detection for Food Safety, State Key Laboratory of Photo catalysis on Energy and Environment, and College of Chemistry, Fuzhou University, Fujian 350108, PR China.
⁵ Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China.
⁶ MOE Key Laboratory of Analysis and Detection for Food Safety, State Key Laboratory of Photo catalysis on Energy and Environment, and College of Chemistry, Fuzhou University, Fujian 350108, PR China. Electronic address: [email protected].

PMID: 27829561
DOI: 10.1016/j.bios.2016.10.026

Abstract

A novel g-C₃N₄ nanosheets embedded with C₃N₄ QDs nanocomposites (QD@CNNS) was prepared by simple oxidation using hydrogen peroxide and UV light irradiation. This nanocomposite exhibits more stable and stronger electrochemiluminescent (ECL) behavior compared with CNNS. Coupling this nanocomposite with Fc-labeled aptamer, a signal-on aptasensor for platelet derived growth factor BB (PDGF-BB) is fabricated. Initially, the Fc-labeled aptamer binds onto QD@CNNS via π-π conjugation and electrostatic interaction, quenching ECL emission from QD@CNNS. The introduction of target efficiently recovers the ECL signal by the formation of PDGF-BB/aptamer complex. The ECL intensity is proportion to the concentration of PDGF-BB in the range of 0.02-80nM with a detection limit of 0.013nM. This work demonstrates a simple synthesis method to obtain QD@CNNS with excellent ECL behavior, and opens up the application of g-C₃N₄ nanocomposite in signal-on aptasensing.

Keywords: C(3)N(4) QDs@ C(3)N(4) nanosheet; Electrochemiluminescent; Platelet derived growth factor BB; Signal-on aptasensor.

Publication types

Evaluation Study

MeSH terms

Aptamers, Nucleotide / chemistry*
Becaplermin
Biosensing Techniques / methods*
Electrochemical Techniques / methods
Humans
Limit of Detection
Luminescent Measurements / methods
Nanocomposites / chemistry
Nanocomposites / ultrastructure
Nanostructures / chemistry*
Nanostructures / ultrastructure
Nitriles / chemistry*
Proto-Oncogene Proteins c-sis / blood*
Quantum Dots / chemistry*
Quantum Dots / ultrastructure

Substances

Aptamers, Nucleotide
Nitriles
Proto-Oncogene Proteins c-sis
Becaplermin
cyanogen