Abstract
The silkworm larvae plasma (SLP) assay has been developed as a means to detect bacterial peptidoglycan as a surrogate for live bacteria. Here, we present results that indicate that generation of melanin by this assay is not fully reliable as a surrogate marker for bacterial count.
Keywords:
Inflammation; Lactobacillus; Pattern recognition receptors.
Published by Elsevier B.V.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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Animals
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Bacteria / isolation & purification*
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Bacterial Load
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Biological Assay*
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Biomarkers / blood
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Bombyx / metabolism*
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DNA, Bacterial / isolation & purification
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Female
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Inflammation / blood*
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Inflammation / microbiology
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Lactobacillus plantarum / isolation & purification
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Larva / metabolism
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Lung / microbiology
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Melanins / blood
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Mice
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Mice, Inbred C57BL
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Peptidoglycan / blood
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Receptors, Pattern Recognition / agonists
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Receptors, Pattern Recognition / metabolism
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Sensitivity and Specificity
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Toll-Like Receptor 2 / genetics
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Toll-Like Receptor 2 / metabolism
Substances
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Biomarkers
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DNA, Bacterial
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Melanins
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Peptidoglycan
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Receptors, Pattern Recognition
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Tlr2 protein, mouse
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Toll-Like Receptor 2