Histological evidence of natural killer cell aggregation against malignant melanoma induced by adoptive immunotherapy with lymphokine-activated killer cells

J Pathol. 1989 Mar;157(3):201-4. doi: 10.1002/path.1711570305.

Abstract

Adoptive immunotherapy with lymphokine-activated killer (LAK) cells and systemic administration of recombinant Interleukin-2 (RIL-2) was carried out in a case of malignant melanoma with lung metastases. Histological specimens from the lung showed a metastatic melanoma heavily invaded by atypical lymphoid cells with convoluted nuclei of varying size. Immunohistochemistry revealed that these cells had the characteristic exclusively of natural killer cell (Leu-7+). Nodules of these cells mimicked the appearance of non-Hodgkin's lymphoma of pleomorphic type. Molecular cytogenetic analysis, however, showed the absence of rearranged bands for the T-cell receptor beta-chain gene, indicating the absence of T-cell clones. At autopsy, 1 month after the LAK therapy, the heavy invasion of convoluted cells had disappeared. These findings clearly indicate that the LAK cell plus RIL-2 therapy induced Leu-7+ lymphoid cells, phenotypically suggestive of natural killer cell aggregation in the tumours.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Aggregation
  • Humans
  • Immunization, Passive*
  • Interleukin-2
  • Killer Cells, Natural / pathology*
  • Lung Neoplasms / pathology
  • Lung Neoplasms / secondary*
  • Lymphokines
  • Male
  • Melanoma / pathology
  • Melanoma / secondary*
  • Middle Aged
  • Skin Neoplasms / pathology*

Substances

  • Interleukin-2
  • Lymphokines