Lack of interspecies barriers in anti-Id stimulated antibody production against Echinococcus granulosus antigens

Parasite Immunol. 1989 Mar;11(2):183-95. doi: 10.1111/j.1365-3024.1989.tb00658.x.

Abstract

Polyclonal human anti-hydatid antibodies were affinity purified from a hydatid patient serum and used to produce a rabbit anti-idiotypic serum. These anti-Id antibodies cross-reacted in ELISA with sera from 11 of 12 hydatid patients studied and with 13 infected or immunized mice sera. All mice primed and boosted with anti-Id produced anti-hydatid antibodies in the primary response and exhibited an increase in antibody titre after a booster injection. The same effect was observed with mice primed with antigen and boosted with anti-Id, although these mice exhibited higher antibody titres. A significant idiotype repertoire is shared by anti-hydatid antibodies produced by different individuals of the same or different species, and anti-Id raised against those antibodies behave as surrogate antigens producing a normal primary and secondary response in animals of different species from that used to isolate the Id.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic
  • Antibodies, Helminth / biosynthesis*
  • Antigens, Helminth*
  • Cross Reactions
  • Echinococcosis / immunology
  • Echinococcosis / prevention & control
  • Echinococcus / immunology*
  • Female
  • Humans
  • Immunization
  • Immunization, Secondary
  • Immunoglobulin Idiotypes
  • Mice
  • Species Specificity

Substances

  • Antibodies, Anti-Idiotypic
  • Antibodies, Helminth
  • Antigens, Helminth
  • Immunoglobulin Idiotypes