Noninvasive assessment of age, gender, and exercise effects on skeletal muscle: Initial experience with T₁ ρ MRI of calf muscle

Purpose: To prospectively investigate age- and gender-related changes in the fast-twitch (tibialis anterior, TA) and slow-twitch (soleus, SOL) skeletal muscle of healthy rats and volunteers and to compare the exercise-related difference in health volunteers with T₁ ρ magnetic resonance imaging (MRI).

Materials and methods: In all, 18 rats and 70 humans were involved in this study. For the animal study, T₁ ρ relaxation times were measured in the TA and SOL rat muscle with a 3.0T MRI scanner and compared to histological data. For the human study, three groups (young, middle-aged, and elderly) of volunteers underwent T₁ ρ MRI scans (3.0T) of their calves. To further differentiate the human scans, 18 volunteers were recruited, half of them (n = 9) routinely trained with high-intensity sports, while the other half (n = 9) with no physical training. Statistical analysis was performed via paired t-test, independent-sample t-test, and analysis of variance (ANOVA). Correlations between T₁ ρ and age/gender/physical endurance were calculated.

Results: The average T₁ ρ relaxation times of the TA and SOL of female rats were higher than that of male rats (P < 0.001). The T₁ ρ relaxation time of TA was significantly lower compared to SOL (P < 0.001). A significant linear correlation was observed between T₁ ρ and the type I slow-twitch fiber proportion (%) in SOL (R² = 0.837, P < 0.001). Similarly, in human studies the average T₁ ρ relaxation times of TA were significantly lower than SOL for all age groups (P < 0.001). The higher T₁ ρ relaxation times of TA and SOL in the elderly volunteers (P < 0.001) and in the females (P < 0.05) indicated significant age- and gender-dependent differences. In high-intensity sports groups, the higher T₁ ρ in SOL (P < 0.01) and lower in TA (P < 0.05) were observed compared with the control group.

Conclusion: This study demonstrated that T₁ ρ MRI can be used to display the differences in fast- and slow-twitch skeletal muscle as well as potentially age-, gender-, and exercise-related differences.

Level of evidence: 2 Technical Efficacy: Stage 1 J. MAGN. RESON. IMAGING 2017;46:61-70.