Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis - Dealing with the immunodiagnosis of meningoencephalitis

Parasitology. 2017 Apr;144(4):459-463. doi: 10.1017/S0031182016001918. Epub 2016 Nov 21.

Abstract

The primary causative agent of eosinophilic meningoencephalitis (EoM) in endemic regions is the nematode Angiostrongylus cantonensis. The occurrence of EoM was previously restricted to countries in Southeast Asia and the Pacific Islands; however, more recently, it has been reported from other regions, including Brazil. The commonly used diagnosis is detection of specific antibody reactivity to the 31 kDa antigen, which is derived from female worm somatic extracts. Here we report the occurrence of cross-reactivity to this antigen in sera from other parasitic infections, especially those that may cause EoM, such as gnathostomiasis, toxocariasis, hydatidosis and strongyloidiasis. We also demonstrated that the cross-reactivity, in part, is dependent of the concentration of antigen used in Western blot assays. We discuss the importance of these findings on the interpretation of this test.

Keywords: 31 kDa antigen; Angiostrongyliasis diagnosis; cross-reactivity; eosinophilic meningoencephalitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiostrongylus cantonensis / immunology*
  • Angiostrongylus cantonensis / metabolism
  • Animals
  • Antigens, Helminth / immunology*
  • Cross Reactions
  • Humans
  • Meningoencephalitis / blood
  • Meningoencephalitis / diagnosis*
  • Meningoencephalitis / parasitology*
  • Strongylida Infections / diagnosis*
  • Strongylida Infections / immunology
  • Strongylida Infections / parasitology

Substances

  • Antigens, Helminth