CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

Alexis C Komor; Ahmed H Badran; David R Liu

doi:10.1016/j.cell.2016.10.044

CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

Cell. 2017 Jan 12;168(1-2):20-36. doi: 10.1016/j.cell.2016.10.044. Epub 2016 Nov 17.

Authors

Alexis C Komor¹, Ahmed H Badran¹, David R Liu²

Affiliations

¹ Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02141, USA.
² Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02141, USA. Electronic address: [email protected].

Abstract

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications. We describe recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems, and we highlight some of the remarkable advancements in basic research, biotechnology, and therapeutics science that these developments have facilitated.

Publication types

Review

MeSH terms

Animals
CRISPR-Cas Systems
Cells, Cultured
Disease / genetics
Disease Models, Animal
Epigenomics / methods
Gene Editing / methods*
Genetic Therapy
Humans

Abstract

Publication types

MeSH terms

Grants and funding