Intracerebroventricular Administration of Amyloid β-protein Oligomers Selectively Increases Dorsal Hippocampal Dialysate Glutamate Levels in the Awake Rat

Sean D O'Shea; Imelda M Smith; Olive M McCabe; Michelle M Cronin; Dominic M Walsh; William T O'Connor

doi:10.3390/s8117428

Intracerebroventricular Administration of Amyloid β-protein Oligomers Selectively Increases Dorsal Hippocampal Dialysate Glutamate Levels in the Awake Rat

Sensors (Basel). 2008 Nov 19;8(11):7428-7437. doi: 10.3390/s8117428.

Authors

Sean D O'Shea¹, Imelda M Smith², Olive M McCabe¹, Michelle M Cronin¹, Dominic M Walsh³, William T O'Connor^{4

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Affiliations

¹ Applied Neurotherapeutics Research Group, Conway Institute, University College Dublin, Dublin 4, Ireland.
² Laboratory for Neurodegenerative Research, Conway Institute, University College Dublin, Dublin 4, Ireland.
³ Laboratory for Neurodegenerative Research, Conway Institute, University College Dublin, Dublin 4, Ireland. [email protected].
⁴ Applied Neurotherapeutics Research Group, Conway Institute, University College Dublin, Dublin 4, Ireland. [email protected].
⁵ Graduate Medical School, University of Limerick, Limerick, Ireland. [email protected].
⁶ Materials and Surface Science Institute, University of Limerick, Limerick, Ireland. [email protected].

Abstract

Extensive evidence supports an important role for soluble oligomers of the amyloid β-protein (Aβ) in Alzheimer's Disease pathogenesis. In the present study we combined intracerebroventricular (icv) injections with brain microdialysis technology in the fully conscious rat to assess the effects of icv administered SDS-stable low-n Aβ oligomers (principally dimers and trimers) on excitatory and inhibitory amino acid transmission in the ipsilateral dorsal hippocampus. Microdialysis was employed to assess the effect of icv administration of Aβ monomers and Aβ oligomers on dialysate glutamate, aspartate and GABA levels in the dorsal hippocampus. Administration of Aβ oligomers was associated with a +183% increase (p.

Keywords: Alzheimer’s Disease; Amyloid β-protein; GABA; Glutamate; Microdialysis; Release.

Abstract

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