Isolation and characterization of adenoviruses infecting endangered golden snub-nosed monkeys (Rhinopithecus roxellana)

Virol J. 2016 Nov 25;13(1):190. doi: 10.1186/s12985-016-0648-6.

Abstract

Background: Adenoviruses are important pathogens with the potential for interspecies transmission between humans and non-human primates. Although many adenoviruses have been identified in monkeys, the knowledge of these viruses from the Colobinae members is quite limited.

Findings: We conducted a surveillance of viral infection in endangered golden snub-nosed monkeys (Rhinopithecus roxellana) in the subfamily Colobinae in China, and found that 5.1% of sampled individuals were positive for adenovirus. One of the adenoviruses (SAdV-WIV19) was successfully isolated and its full-length genome was sequenced. The full-length genome of WIV19 is 33,562 bp in size, has a G + C content of 56.2%, and encodes 35 putative genes. Sequence analysis revealed that this virus represents a novel species in the genus Mastadenovirus. Diverse cell lines, including those of human origin, were susceptible to WIV19.

Conclusion: We report the first time the isolation and full-length genomic characterization of an adenovirus from the subfamily Colobinae.

Keywords: Golden snub-nosed monkey; Mastadenovirus; Simian adenovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / classification*
  • Adenoviridae / genetics
  • Adenoviridae / isolation & purification*
  • Adenoviridae Infections / epidemiology
  • Adenoviridae Infections / veterinary*
  • Adenoviridae Infections / virology
  • Animals
  • Base Composition
  • China / epidemiology
  • Cluster Analysis
  • Colobinae / virology*
  • Gene Order
  • Genes, Viral
  • Genome, Viral
  • Phylogeny
  • Prevalence
  • Primate Diseases / epidemiology*
  • Primate Diseases / virology*
  • Sequence Analysis, DNA