Phenotypic and genomic characterization of the antimicrobial producer Rheinheimera sp. EpRS3 isolated from the medicinal plant Echinacea purpurea: insights into its biotechnological relevance

Res Microbiol. 2017 Apr;168(3):293-305. doi: 10.1016/j.resmic.2016.11.001. Epub 2016 Nov 22.

Abstract

In recent years, there has been increasing interest in plant microbiota; however, despite medicinal plant relevance, very little is known about their highly complex endophytic communities. In this work, we report on the genomic and phenotypic characterization of the antimicrobial compound producer Rheinheimera sp. EpRS3, a bacterial strain isolated from the rhizospheric soil of the medicinal plant Echinacea purpurea. In particular, EpRS3 is able to inhibit growth of different bacterial pathogens (Bcc, Acinetobacter baumannii, and Klebsiella pneumoniae) which might be related to the presence of gene clusters involved in the biosynthesis of different types of secondary metabolites. The outcomes presented in this work highlight the fact that the strain possesses huge biotechnological potential; indeed, it also shows antimicrobial effects upon well-described multidrug-resistant (MDR) human pathogens, and it affects plant root elongation and morphology, mimicking indole acetic acid (IAA) action.

Keywords: Antibiotic production; Antibiotic resistance; Biotechnological applications; Endophytes; Genome analysis.

MeSH terms

  • Acinetobacter baumannii / growth & development
  • Anti-Bacterial Agents / pharmacology
  • Antibiosis*
  • Biotechnology
  • Burkholderia cepacia / growth & development
  • Echinacea / microbiology*
  • Endophytes / isolation & purification
  • Fosfomycin / pharmacology
  • Gammaproteobacteria / chemistry
  • Gammaproteobacteria / genetics*
  • Gammaproteobacteria / isolation & purification
  • Gammaproteobacteria / physiology*
  • Genomics
  • Indoleacetic Acids / metabolism
  • Klebsiella pneumoniae / growth & development
  • Phenotype
  • Plant Roots / microbiology
  • Plants, Medicinal / microbiology
  • Rhizosphere*

Substances

  • Anti-Bacterial Agents
  • Indoleacetic Acids
  • Fosfomycin
  • indoleacetic acid