Increased sclerostin and bone turnover after diet-induced weight loss in type 2 diabetes: a post hoc analysis of the MADIAB trial

Endocrine. 2017 Jun;56(3):667-674. doi: 10.1007/s12020-016-1171-7. Epub 2016 Nov 25.

Abstract

Background: Sclerostin has been directly related to bone turnover increase in dietary-induced weight loss in non-diabetics. This has not been studied in type 2 diabetes, a condition characterized by increased circulating sclerostin and impaired bone turnover.

Purpose: To study the effect of dietary weight loss and quality of the dietary intervention on changes of sclerostin and bone turnover markers in type 2 diabetes.

Methods: This was a post-hoc analysis of the MADIAB trial, a 21-day randomized controlled trial on overweight/obese type 2 diabetes patients. Patients were randomly assigned 1:1 to the Ma-Pi2 macrobiotic diet or a control diet based on dietary guidelines for type 2 diabetes. Serum sclerostin and circulating markers of bone resorption and formation (P1NP) were measured by enzyme linked immunosorbent assay in 40 subjects (1:1) at baseline and after 21 days treatment.

Results: Both Ma-Pi2 and the control diet groups had significant decreases in body weight (6.0 ± 0.2 vs. 3.2 ± 0.1 %, p < 0.001). Sclerostin increased significantly in the two groups (all p < 0.001) but Ma-Pi2 diet group experienced a greater increase in sclerostin (34.5 vs. 15 %; p = 0.024). Serum circulating markers of bone resorption increased in the two groups (all p < 0.001); circulating markers of bone resorption at the end of the treatment tended to be higher in Ma-Pi2 diet than the control diet group (p = 0.06). P1NP did not change significantly in the two group compared to baseline. Sclerostin changes were related to body mass index reduction (r = -0.37; p = 0.02).

Conclusions: Diet-induced weight loss may induce significant and rapid changes in bone turnover and sclerostin levels. These changes may further impair bone health in subjects with type 2 diabetes.

Keywords: Bone turnover; Macrobiotic Ma-Pi2 diet; Type 2 diabetes.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Aged
  • Biomarkers / blood
  • Body Weight / physiology
  • Bone Morphogenetic Proteins / blood*
  • Bone Remodeling / physiology*
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / physiopathology
  • Diet, Reducing*
  • Female
  • Genetic Markers
  • Humans
  • Male
  • Middle Aged
  • Obesity / blood
  • Obesity / diet therapy
  • Obesity / physiopathology
  • Overweight / blood*
  • Overweight / diet therapy
  • Overweight / physiopathology
  • Treatment Outcome
  • Weight Loss / physiology*

Substances

  • Adaptor Proteins, Signal Transducing
  • Biomarkers
  • Bone Morphogenetic Proteins
  • Genetic Markers
  • SOST protein, human