The utility of direct specimen detection by Sanger sequencing in hospitalized pediatric patients

Kanokporn Mongkolrattanothai; Jennifer Dien Bard

doi:10.1016/j.diagmicrobio.2016.10.024

The utility of direct specimen detection by Sanger sequencing in hospitalized pediatric patients

Diagn Microbiol Infect Dis. 2017 Feb;87(2):100-102. doi: 10.1016/j.diagmicrobio.2016.10.024. Epub 2016 Nov 2.

Authors

Kanokporn Mongkolrattanothai¹, Jennifer Dien Bard²

Affiliations

¹ Division of Infectious Diseases, Department of Pediatrics, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033, USA. Electronic address: [email protected].
² Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033, USA.

PMID: 27889254
DOI: 10.1016/j.diagmicrobio.2016.10.024

Abstract

Direct microbial DNA detection from clinical specimens by polymerase chain reaction and Sanger sequencing has been developed to address the innate limitations of traditional culture-based work-up. We report our institution's experience with direct specimen sequencing, its clinical utility, and barriers to effective clinical implementation.

Keywords: Direct specimen sequencing.

Publication types

Evaluation Study

MeSH terms

Adolescent
Bacterial Infections / diagnosis*
Bacterial Infections / microbiology
Child
Child, Preschool
Humans
Infant
Molecular Diagnostic Techniques / methods*
Mycoses / diagnosis*
Mycoses / microbiology
Polymerase Chain Reaction / methods*
Sequence Analysis, DNA / methods*