Cellular Aging Contributes to Failure of Cold-Induced Beige Adipocyte Formation in Old Mice and Humans

Cell Metab. 2017 Jan 10;25(1):166-181. doi: 10.1016/j.cmet.2016.10.023. Epub 2016 Nov 23.

Abstract

Cold temperatures induce progenitor cells within white adipose tissue to form beige adipocytes that burn energy and generate heat; this is a potential anti-diabesity therapy. However, the potential to form cold-induced beige adipocytes declines with age. This creates a clinical roadblock to potential therapeutic use in older individuals, who constitute a large percentage of the obesity epidemic. Here we show that aging murine and human beige progenitor cells display a cellular aging, senescence-like phenotype that accounts for their age-dependent failure. Activating the senescence pathway, either genetically or pharmacologically, in young beige progenitors induces premature cellular senescence and blocks their potential to form cold-induced beige adipocytes. Conversely, genetically or pharmacologically reversing cellular aging by targeting the p38/MAPK-p16Ink4a pathway in aged mouse or human beige progenitor cells rejuvenates cold-induced beiging. This in turn increases glucose sensitivity. Collectively, these data indicate that anti-aging or senescence modalities could be a strategy to induce beiging, thereby improving metabolic health in aging humans.

Keywords: Ink4a/Arf; adipose; beige adipocytes; cellular aging; cold exposure; mural cells; senescence; thermogenesis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Actins / metabolism
  • Adipocytes, Beige / cytology*
  • Adipocytes, Beige / metabolism*
  • Aging / physiology*
  • Animals
  • Cellular Senescence*
  • Cold Temperature*
  • Cyclin-Dependent Kinase Inhibitor p16 / metabolism
  • Humans
  • Male
  • Mice, Inbred C57BL
  • Phenotype
  • Stem Cells / metabolism
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Actins
  • Cyclin-Dependent Kinase Inhibitor p16
  • alpha-smooth muscle actin, mouse
  • p38 Mitogen-Activated Protein Kinases