Host-pathogen protein-protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host-pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.
Keywords: Bacteria; Bioinformatics; Mass spectrometry; Peptide solid phase extraction; Proteomics; Surface absorption; Trypsin digestion.