Generation and characterization of an induced pluripotent stem cell (iPSC) line from a patient with clozapine-resistant Schizophrenia

Stem Cell Res. 2016 Nov;17(3):661-664. doi: 10.1016/j.scr.2016.11.005. Epub 2016 Nov 10.

Abstract

Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as "super-refractory") Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antipsychotic Agents / therapeutic use*
  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming
  • Clozapine / therapeutic use*
  • Embryoid Bodies / cytology
  • Embryoid Bodies / metabolism
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Leukocytes, Mononuclear / cytology
  • Male
  • Microscopy, Fluorescence
  • Schizophrenia / drug therapy*
  • Schizophrenia / metabolism
  • Schizophrenia / pathology
  • Sendai virus / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Antipsychotic Agents
  • Transcription Factors
  • Clozapine