Sinu virus, a novel and divergent orthomyxovirus related to members of the genus Thogotovirus isolated from mosquitoes in Colombia

Virology. 2017 Jan 15:501:166-175. doi: 10.1016/j.virol.2016.11.014. Epub 2016 Dec 6.

Abstract

The genome and structural organization of a novel insect-specific orthomyxovirus, designated Sinu virus, is described. Sinu virus (SINUV) was isolated in cultures of C6/36 cells from a pool of mosquitoes collected in northwestern Colombia. The virus has six negative-sense ssRNA segments. Genetic analysis of each segment demonstrated the presence of six distinct ORFs encoding the following genes: PB2 (Segment 1), PB1, (Segment 2), PA protein (Segment 3), envelope GP gene (Segment 4), the NP (Segment 5), and M-like gene (Segment 6). Phylogenetically, SINUV appears to be most closed related to viruses in the genus Thogotovirus.

Keywords: Insect-specific viruses; Orthomyxoviridae; Sinu virus; Thogotovirus genus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Colombia
  • Culicidae / virology*
  • Evolution, Molecular*
  • Genome, Viral
  • Models, Molecular
  • Molecular Sequence Data
  • Orthomyxoviridae / chemistry
  • Orthomyxoviridae / classification
  • Orthomyxoviridae / genetics
  • Orthomyxoviridae / isolation & purification*
  • Phylogeny
  • Thogotovirus / chemistry
  • Thogotovirus / classification
  • Thogotovirus / genetics
  • Thogotovirus / isolation & purification
  • Viral Proteins / chemistry
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • Viral Proteins