Household Consumption of Thiamin-Fortified Fish Sauce Increases Erythrocyte Thiamin Concentrations among Rural Cambodian Women and Their Children Younger Than 5 Years of Age: A Randomized Controlled Efficacy Trial

J Pediatr. 2017 Feb:181:242-247.e2. doi: 10.1016/j.jpeds.2016.10.066. Epub 2016 Dec 7.

Abstract

Objectives: To assess whether ad libitum consumption of thiamin-fortified fish sauce over 6 months yields higher erythrocyte thiamin diphosphate concentrations (eTDP) among women of childbearing age and their children aged 12-59 months compared with control sauce containing no thiamin.

Study design: In this double-blind, randomized controlled efficacy trial, 276 nonpregnant, nonlactating women (18-45 years of age) and their families in Prey Veng, Cambodia, were randomized to receive 1 of 3 fish sauce formulations: low thiamin concentration (low, 2 g/L), high thiamin concentration (high, 8 g/L), or a control (no thiamin) fish sauce. Baseline (t = 0) and endline (t = 6 months) eTDP were measured with the use of high-performance liquid chromatography with a fluorescence detector.

Results: Fish sauce consumption did not differ between treatment groups (P = .19). In intent-to-treat analysis, women's baseline-adjusted endline eTDP (mean; 95% CI) was higher among women in the low (259; 245-274 nmol/L) and high (257; 237-276 nmol/L) groups compared with control (184; 169-198 nmol/L; P < .001); low and high groups did not differ (P = .83). Similarly, children's baseline-adjusted eTDP was higher in the low (259; 246-271 nmol/L) and high (257; 243-270 nmol/L) groups compared with control (213; 202-224 nmol/L; P < .001).

Conclusion: Fortified fish sauce appears to be an efficacious means of improving biochemical thiamin status in nonpregnant, nonlactating women and their children (1-5 years of age) living in rural Cambodia.

Trial registration: ClinicalTrials.gov: NCT02221063.

Keywords: Southeast Asia; fish sauce; fortification; maternal and child health; thiamin.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Cambodia
  • Child
  • Child, Preschool
  • Chromatography, Liquid
  • Double-Blind Method
  • Erythrocytes / metabolism*
  • Female
  • Fish Products*
  • Food, Fortified*
  • Humans
  • Infant
  • Middle Aged
  • Nutritional Status
  • Rural Population
  • Thiamine / administration & dosage*
  • Thiamine / blood
  • Young Adult

Substances

  • Thiamine

Associated data

  • ClinicalTrials.gov/NCT02221063

Grants and funding