A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice

Rui Yang; Yafei Li; Yan Su; Yi Shen; Ding Tang; Qiong Luo; Zhukuan Cheng

doi:10.1016/j.jgg.2016.09.006

A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice

J Genet Genomics. 2016 Dec 20;43(12):694-701. doi: 10.1016/j.jgg.2016.09.006. Epub 2016 Oct 13.

Authors

Rui Yang¹, Yafei Li², Yan Su², Yi Shen², Ding Tang², Qiong Luo³, Zhukuan Cheng⁴

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China.
² State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
³ State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China. Electronic address: [email protected].
⁴ State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: [email protected].

PMID: 27965027
DOI: 10.1016/j.jgg.2016.09.006

Abstract

An awned rice (Oryza sativa) plant carrying a tiny extra chromosome was discovered among the progeny of a telotrisomic line 2n+4L. Fluorescence in situ hybridization (FISH) using chromosome specific BAC clones revealed that this extra chromosome was a ring chromosome derived from part of the long arm of chromosome 4. So the aneuploidy plant was accordingly named as 2n+4L ring. We did not detect any CentO FISH signals on the ring chromosome, and found only the centromeric probe Centromeric Retrotransposon of Rice (CRR) was co-localized with the centromere-specific histone CENH3 as revealed by sequential FISH after immunodetection. The extra ring chromosome exhibited a unique segregation pattern during meiosis, including no pairing between the ring chromosome and normal chromosome 4 during prophase I and pre-separation of sister chromatids at anaphase I.

Keywords: Centromere; Meiosis; Rice; Ring chromosome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Centromere / genetics*
Meiosis / genetics
Mutation
Oryza / cytology
Oryza / genetics*
Oryza / physiology
Reproduction
Ring Chromosomes*
Tandem Repeat Sequences*