Glucagon-like Peptide 1 Receptor Signaling in Acinar Cells Causes Growth-Dependent Release of Pancreatic Enzymes

Cell Rep. 2016 Dec 13;17(11):2845-2856. doi: 10.1016/j.celrep.2016.11.051.

Abstract

Incretin-based therapies are widely used for type 2 diabetes and now also for obesity, but they are associated with elevated plasma levels of pancreatic enzymes and perhaps a modestly increased risk of acute pancreatitis. However, little is known about the effects of the incretin hormone glucagon-like peptide 1 (GLP-1) on the exocrine pancreas. Here, we identify GLP-1 receptors on pancreatic acini and analyze the impact of receptor activation in humans, rodents, isolated acini, and cell lines from the exocrine pancreas. GLP-1 did not directly stimulate amylase or lipase release. However, we saw that GLP-1 induces phosphorylation of the epidermal growth factor receptor and activation of Foxo1, resulting in cell growth with concomitant enzyme release. Our work uncovers GLP-1-induced signaling pathways in the exocrine pancreas and suggests that increases in amylase and lipase levels in subjects treated with GLP-1 receptor agonists reflect adaptive growth rather than early-stage pancreatitis.

Keywords: GLP-1; GLP-1R; acinar cells; amylase; lipase; pancreatic enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinar Cells / drug effects
  • Acinar Cells / enzymology
  • Amylases / genetics*
  • Animals
  • Cell Line
  • Cell Proliferation / drug effects
  • Diabetes Mellitus, Type 2 / drug therapy
  • Diabetes Mellitus, Type 2 / enzymology
  • Forkhead Box Protein O1 / genetics*
  • Gene Expression Regulation, Enzymologic
  • Glucagon-Like Peptide 1 / administration & dosage
  • Glucagon-Like Peptide 1 / genetics*
  • Glucagon-Like Peptide-1 Receptor / agonists
  • Glucagon-Like Peptide-1 Receptor / genetics*
  • Humans
  • Incretins / therapeutic use
  • Lipase / genetics*
  • Pancreas / enzymology
  • Pancreatitis / chemically induced
  • Pancreatitis / genetics
  • Pancreatitis / pathology
  • Signal Transduction

Substances

  • Forkhead Box Protein O1
  • Glucagon-Like Peptide-1 Receptor
  • Incretins
  • Glucagon-Like Peptide 1
  • Lipase
  • Amylases