Proteomic investigation of human cystic echinococcosis in the liver

Rémi Longuespée; Rita Casadonte; Mark Kriegsmann; Petra Wandernoth; Katharina Lisenko; Gabriel Mazzucchelli; Michael Becker; Jörg Kriegsmann

doi:10.1016/j.molbiopara.2016.12.002

Proteomic investigation of human cystic echinococcosis in the liver

Mol Biochem Parasitol. 2017 Jan:211:9-14. doi: 10.1016/j.molbiopara.2016.12.002. Epub 2016 Dec 13.

Authors

Rémi Longuespée¹, Rita Casadonte², Mark Kriegsmann³, Petra Wandernoth⁴, Katharina Lisenko⁵, Gabriel Mazzucchelli⁶, Michael Becker⁷, Jörg Kriegsmann⁸

Affiliations

¹ Proteopath GmbH, Trier, Max Planck Strasse 17, 54296 Trier, Germany; Institute of Pathology, University of Heidelberg, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany. Electronic address: [email protected].
² Proteopath GmbH, Trier, Max Planck Strasse 17, 54296 Trier, Germany.
³ Institute of Pathology, University of Heidelberg, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany.
⁴ Molecular Pathology Trier, Max Planck Strasse 17, 54296 Trier, Germany.
⁵ Department of Internal Medicine V, University of Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
⁶ Mass Spectrometry Laboratory (LSM), Systems Biology and Chemical Biology, GIGA-Research, University of Liège, Allée du 6 aoÛt 11, 4000 Liège, Belgium.
⁷ Bruker Daltonik GmbH, Bremen, Germany, Fahrenheitstrasse, 28359 Bremen, Germany.
⁸ Proteopath GmbH, Trier, Max Planck Strasse 17, 54296 Trier, Germany; Molecular Pathology Trier, Max Planck Strasse 17, 54296 Trier, Germany; MVZ for Histology, Cytology and Molecular Diagnostics Trier, Max Planck Strasse 5, 54296 Trier, Germany.

PMID: 27986452
DOI: 10.1016/j.molbiopara.2016.12.002

Abstract

Cystic echinococcosis (CE) is a pandemic infectious disease caused by the tapeworm Echinococcus granulosus that forms cysts in different organs such as lungs and liver. Imaging examination and serological tests have some drawbacks such as low sensitivity. In this study, we used an up-to-date workflow of laser microdissection-based microproteomics and matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry in order to depict the proteomic pattern of CE in the liver. This investigation revealed specific markers of a parasitic cyst in liver. This proteomic pattern could facilitate diagnosis of CE in the future.

Keywords: Cystic echinococcosis; Histoproteomics; Laser microdissection; MALDI IMS; Parasitic disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, Liquid
Echinococcosis, Hepatic / metabolism*
Echinococcosis, Hepatic / parasitology*
Echinococcosis, Hepatic / pathology
Echinococcus granulosus*
Humans
Laser Capture Microdissection
Proteome*
Proteomics* / methods
Tandem Mass Spectrometry

Substances

Proteome