Emergence of sequence type 11 Klebsiella pneumoniae coproducing NDM-1 and VIM-1 metallo-β-lactamases in a Greek hospital

C C Papagiannitsis; E Malli; Z Florou; S Sarrou; J Hrabak; K Mantzarlis; E Zakynthinos; E Petinaki

doi:10.1016/j.diagmicrobio.2016.12.008

Emergence of sequence type 11 Klebsiella pneumoniae coproducing NDM-1 and VIM-1 metallo-β-lactamases in a Greek hospital

Diagn Microbiol Infect Dis. 2017 Mar;87(3):295-297. doi: 10.1016/j.diagmicrobio.2016.12.008. Epub 2016 Dec 14.

Authors

C C Papagiannitsis¹, E Malli², Z Florou², S Sarrou², J Hrabak³, K Mantzarlis⁴, E Zakynthinos⁴, E Petinaki⁵

Affiliations

¹ Department of Microbiology, University Hospital of Larissa, Larissa, Greece; Biomedical Center, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic.
² Department of Microbiology, University Hospital of Larissa, Larissa, Greece.
³ Biomedical Center, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic.
⁴ Department of Critical Care, Medical School, University of Thessaly, Larissa, Greece.
⁵ Department of Microbiology, University Hospital of Larissa, Larissa, Greece. Electronic address: [email protected].

PMID: 27993422
DOI: 10.1016/j.diagmicrobio.2016.12.008

Abstract

Sequence type 11 Klebsiella pneumoniae, coproducing NDM-1 and VIM-1 metallo-β-lactamases, were isolated in a Greek hospital. bla_NDM-1 was part of a Tn125 derivative, located on an ~90-kb plasmid similar to the NDM-1-encoding plasmid pB-3002cz. bla_VIM-1 was located in an In-e541-like integron, carried on a multireplicon (IncA/C and IncR) plasmid of ~180kb.

Keywords: Carbapenemases; Colonization; Enterobacteriaceae; NDM-1; VIM-1.

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Carbapenems / pharmacology*
Greece
Hospitals
Humans
Klebsiella Infections / drug therapy*
Klebsiella Infections / microbiology
Klebsiella pneumoniae / classification
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Bacterial Proteins
Carbapenems
VIM-1 metallo-beta-lactamase
beta-Lactamases
beta-lactamase NDM-1
carbapenemase