Malagasy Conostigmus (Hymenoptera: Ceraphronoidea) and the secret of scutes

PeerJ. 2016 Dec 13:4:e2682. doi: 10.7717/peerj.2682. eCollection 2016.

Abstract

We revise the genus Conostigmus Dahlbom 1858 occurring in Madagascar, based on data from more specimens than were examined for the latest world revision of the genus. Our results yield new information about intraspecific variability and the nature of the atypical latitudinal diversity gradient (LDG) observed in Ceraphronoidea. We also investigate cellular processes that underlie body size polyphenism, by utilizing the correspondence between epidermal cells and scutes, polygonal units of leather-like microsculpture. Our results reveal that body size polyphenism in Megaspilidae is most likely related to cell number and not cell size variation, and that cell size differs between epithelial fields of the head and that of the mesosoma. Three species, Conostigmus ballescoracas Dessart, 1997, C. babaiax Dessart, 1996 and C. longulus Dessart, 1997, are redescribed. Females of C. longulus are described for the first time, as are nine new species: C. bucephalus Mikó and Trietsch sp. nov., C. clavatus Mikó and Trietsch sp. nov., C. fianarantsoaensis Mikó and Trietsch sp. nov., C. lucidus Mikó and Trietsch sp. nov., C. macrocupula, Mikó and Trietsch sp. nov., C. madagascariensis Mikó and Trietsch sp. nov., C. missyhazenae Mikó and Trietsch sp. nov., C. pseudobabaiax Mikó and Trietsch sp. nov., and C. toliaraensis Mikó and Trietsch sp. nov. A fully illustrated identification key for Malagasy Conostigmus species and a Web Ontology Language (OWL) representation of the taxonomic treatment, including specimen data, nomenclature, and phenotype descriptions, in both natural and formal languages, are provided.

Keywords: CLSM; Imaginal disks; LDG; Male genitalia; Microscopy; Morphology; Phenotypic plasticity; Taxonomy.

Grants and funding

This material is based upon work supported by the U.S. National Science Foundation, under Grant Nos. DBI-0850223, DEB-0956049, DBI-1356381, DEB-1353252 and Grant No. DEB-0072713 to B.L. Fisher and C.E. Griswold and Grant No. DEB-0344731 to B.L. Fisher and P.S. Ward. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.