Plasmids of Distinct IncK Lineages Show Compatible Phenotypes

Marta Rozwandowicz; Michael S M Brouwer; Aldert L Zomer; Alex Bossers; Frank Harders; Dik J Mevius; Jaap A Wagenaar; Joost Hordijk

doi:10.1128/AAC.01954-16

Plasmids of Distinct IncK Lineages Show Compatible Phenotypes

Antimicrob Agents Chemother. 2017 Feb 23;61(3):e01954-16. doi: 10.1128/AAC.01954-16. Print 2017 Mar.

Authors

Marta Rozwandowicz¹, Michael S M Brouwer², Aldert L Zomer¹, Alex Bossers², Frank Harders², Dik J Mevius^{1

2}, Jaap A Wagenaar^{1

2}, Joost Hordijk³

Affiliations

¹ Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
² Wageningen Bioveterinary Research, Lelystad, The Netherlands.
³ Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands [email protected].

Abstract

IncK plasmids are some of the main carriers of bla_CTX-M-14 and bla_CMY-2 genes and show high similarity to other plasmids belonging to the I complex, including IncB/O plasmids. Here, we studied the phylogenetic relationship of 37 newly sequenced IncK and IncB/O plasmids. We show that IncK plasmids can be divided into two compatible lineages named IncK1 and IncK2.

Keywords: IncK; incompatibility; plasmid.

MeSH terms

Conjugation, Genetic
Escherichia coli / classification
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / microbiology
Escherichia coli Proteins / genetics*
Escherichia coli Proteins / metabolism
Gene Expression
Humans
Phenotype*
Phylogeny*
Plasmids / chemistry
Plasmids / classification*
Plasmids / metabolism
Sequence Analysis, DNA
Transformation, Bacterial
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Escherichia coli Proteins
beta-lactamase CTX-M-14, E coli
beta-Lactamases
beta-lactamase CMY-2, E coli