Abstract
The identification of conventional dendritic cells (cDCs) and macrophages (mϕ) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from each other and from other intestinal myeloid cells. Here we detail the protocols we have developed to isolate live leukocytes from steady state mouse small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs, monocytes and macrophages in such preparations.
Keywords:
Dendritic cells; Identification; Intestine; Isolation; Macrophages; Subsets.
MeSH terms
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Animals
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Antibodies / chemistry
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Antigens, CD / genetics
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Antigens, CD / immunology
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Biomarkers / metabolism
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Cell Separation / instrumentation
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Cell Separation / methods*
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Collagenases / chemistry
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Colon / cytology*
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Colon / immunology
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Dendritic Cells / cytology
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Dendritic Cells / immunology
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Deoxyribonucleases / chemistry
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Endopeptidases / chemistry
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Flow Cytometry / methods*
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Gene Expression
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Intestine, Small / cytology*
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Intestine, Small / immunology
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Macrophages / cytology
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Macrophages / immunology
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Mice
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Monocytes / cytology
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Monocytes / immunology
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Staining and Labeling / methods*
Substances
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Antibodies
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Antigens, CD
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Biomarkers
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Deoxyribonucleases
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Endopeptidases
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Collagenases
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dispase