Draft genome sequence of an aminoglycoside-resistant RmtG-producing Pseudomonas aeruginosa ST235 isolated from a cystic fibrosis patient

Maria Fernanda C Bueno; Gabriela Rodrigues Francisco; Louise Cerdeira; Susan Ienne; Tiago A Souza; Nilton Lincopan; Doroti de Oliveira Garcia

doi:10.1016/j.jgar.2016.11.005

Draft genome sequence of an aminoglycoside-resistant RmtG-producing Pseudomonas aeruginosa ST235 isolated from a cystic fibrosis patient

J Glob Antimicrob Resist. 2017 Mar:8:106-107. doi: 10.1016/j.jgar.2016.11.005. Epub 2017 Jan 7.

Authors

Maria Fernanda C Bueno¹, Gabriela Rodrigues Francisco¹, Louise Cerdeira², Susan Ienne³, Tiago A Souza³, Nilton Lincopan⁴, Doroti de Oliveira Garcia⁵

Affiliations

¹ Center of Bacteriology, Instituto Adolfo Lutz, São Paulo, Brazil.
² Department of Clinical Analysis, School of Pharmacy, Universidade de São Paulo, São Paulo, Brazil.
³ Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil.
⁴ Department of Clinical Analysis, School of Pharmacy, Universidade de São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil.
⁵ Center of Bacteriology, Instituto Adolfo Lutz, São Paulo, Brazil. Electronic address: [email protected].

PMID: 28082143
DOI: 10.1016/j.jgar.2016.11.005

Abstract

Cystic fibrosis (CF) patients are often chronically colonised or infected by non-fermenting Gram-negative bacilli, with Pseudomonas aeruginosa being the most prevalent. In this study, we report the draft genome sequence of a multidrug-resistant P. aeruginosa strain belonging to sequence type ST235, isolated from the respiratory tract of a CF patient with chronic colonisation. Whole-genome sequencing analysis revealed a 6.7Mb genome size and the presence of 12 antibiotic resistance genes, including the rmtG gene conferring high-level aminoglycoside resistance, located on the chromosome.

Keywords: Brazil; Cystic fibrosis; Pseudomonas aeruginosa; RmtG; WGS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aminoglycosides / pharmacology
Anti-Bacterial Agents / pharmacology
Cystic Fibrosis / complications
Drug Resistance, Bacterial
Genes, Bacterial
Genome, Bacterial*
Humans
Pseudomonas Infections / microbiology
Pseudomonas aeruginosa / drug effects
Pseudomonas aeruginosa / genetics*
Pseudomonas aeruginosa / isolation & purification
Sequence Analysis, DNA*
Whole Genome Sequencing*

Substances

Aminoglycosides
Anti-Bacterial Agents