In vivo and in silico sedative-hypnotic like activity of 7-methyljuglone isolated from Diospyros lotus L

Abdur Rauf; Sengul Uysal; Taibi Ben Hadda; Ghias Uddin; Muhammad Asif Nawaz; Haroon Khan; Bina S Siddiqui; Muslim Raza; Saud Bawazeer; Gokhan Zengin

doi:10.1016/j.biopha.2017.01.021

In vivo and in silico sedative-hypnotic like activity of 7-methyljuglone isolated from Diospyros lotus L

Biomed Pharmacother. 2017 Mar:87:678-682. doi: 10.1016/j.biopha.2017.01.021. Epub 2017 Jan 12.

Authors

Affiliations

¹ Department of Chemistry, University of Swabi, Swabi, Anbar, 23561 Khyber Pakhtunkhwa, Pakistan. Electronic address: [email protected].
² Deparment of Biology, University of Selcuk, Konya, Turkey.
³ LCMS laboratory, Univesity of Mohamed 1st, Faculty of Sciences, Oujda 60000, Morocco.
⁴ Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan.
⁵ Department of Bio-Technology, Shaheed Benazir Bhutto University Sheringal Dir (Upper), KP, Pakistan.
⁶ Department of Pharmacy, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
⁷ H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
⁸ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
⁹ Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, P.O. Box 42, Saudi Arabia.

PMID: 28088734
DOI: 10.1016/j.biopha.2017.01.021

Abstract

Diospyros lotus L. possesses different therapeutic activities such as antioxidant, anti-proliferative, anti-microbial and sedative. However, no studies on the sedative-hypnotic activity of 7-methyljuglone are reported. In the present study, we have evaluated in vivo the anxiolytic-hypnotic like effects of 7-methyljuglone in mice with open field and phenobarbitone-induced sleeping time tests. We have also assessed in silico the involvement of GABA_A, GABA_B and 5HT1 neurotransmission in its mechanism of action. The intraperitoneal administration of 7-methyljuglone (2.5-10mg/kg) reduce significantly the number of crossed lines in mice open field test and concomitantly it shown a significant activity in term of onset of sleeping time and also in its duration. Moreover, 7-methyljuglone demonstrated in silico an interesting interaction with GABA_A but not GABA_B and 5HT1binding sites. All of these results, taken together, 7-methyljuglone may be an innovative candidate for designing new pharmaceutical and therapeutic applications.

Keywords: 7-methyljuglone; Diospyros lotus L.; Docking study; Gaba; Medicinal plants; Sedative-hypnotic activity.

MeSH terms

Animals
Antioxidants / metabolism
Diospyros / chemistry*
Hypnotics and Sedatives / pharmacology*
Male
Mice
Mice, Inbred BALB C
Naphthoquinones / pharmacology*
Plant Extracts / pharmacology*

Substances

7-methyljuglone
Antioxidants
Hypnotics and Sedatives
Naphthoquinones
Plant Extracts